Microbiome Insights Sample Report:
Quantitative PCR (qPCR) Analysis
When you request a Quantitative PCR (qPCR) analysis from Microbiome Insights, our laboratory team delivers precise target quantification that lets you see critical biological metrics across your study groups at a glance. We provide your raw datasets alongside an interactive HTML report designed to evaluate targeted microbial abundances, study metadata, and statistical significance.
Depending on your study design and custom metadata, our qPCR reports deliver:
- Absolute Quantification Summaries: Exact target abundance per sample (e.g., bacterial copies per mg of sample) mapped directly to your sample IDs and metadata.
- Statistical Modeling & Hypotheses Testing: We use your experimental design to put the results in the experimental context. From cross-sectional to longitudinal analysis, we work with you in consultation calls to understand your approach and avoid problems later.
- Comprehensive QC & Assay Curves: Full transparency into assay performance, featuring raw amplification RFU curves, melt curve specificity checks, and standard curves with explicit efficiency, and slope calculations.
- Standardized Laboratory Protocols: Detailed methodological documentation covering extraction parameters (e.g., Macherey-Nagel NucleoMag DNA Microbiome kit on KingFisher robots), primer specifications (e.g., 27F/519R 16S rRNA target primers), and thermocycling profiles.
Your data is stored securely on Microbiome Insights’ on-site servers in British Columbia, Canada, and transferred directly to you via our encrypted Nextcloud platform. Reports are delivered as fully responsive HTML files for seamless exploration.
Complete the form below to receive a sample qPCR report showcasing our absolute quantification workflows, statistical models, and quality metrics.