Microbiome Insights Sample Report:

ITS2 Fungal Amplicon Analysis 

When you request a fungal ITS2 (Internal Transcribed Spacer 2) analysis from Microbiome Insights, our lab team delivers detailed characterization of the mycobiome in your samples. We provide raw datasets alongside an interactive HTML report to help you visualize fungal community structure, taxonomic composition, and differential shifts across your study groups.

Depending on your study design and custom metadata, our ITS2 sample reports showcase:

  • Sequence Curation & Quality Metrics: Quality evaluation summaries (e.g., FastQC), read-count distribution plots, and downloadable quality-filtered sample mapping tables.
  • Taxonomic Composition & Aggregation: Interactive relative abundance profiles aggregated from phylum to species levels (e.g., Basidiomycota, Ascomycota, Malassezia, Cladosporium), alongside summary tables of top abundant taxa by group.
  • Alpha & Beta Diversity Analysis: Comprehensive profiling of within-sample diversity and community composition tailored to your experimental design—including flexible multi-factor and longitudinal modeling (e.g., Linear Mixed Models), coupled with principal coordinate ordination (PCoA) and multivariate significance testing (PERMANOVA).
  • Differential Abundance & Biomarker Discovery: Multivariable modeling via MaAsLin3 to identify significantly enriched fungal features, complete with post-hoc contrast tables and interactive log-scaled species boxplots.
  • Standardized Mycobiome Protocols: Comprehensive documentation covering extraction methods (Macherey-Nagel NucleoMag DNA Microbiome kit on KingFisher robots), dual-indexed ITS3/ITS4 priming on the Illumina MiSeq platform, and OTU clustering against the UNITE (v10) database using mothur.

Your data is stored securely on Microbiome Insights’ on-site servers in British Columbia, Canada, and transferred directly via our encrypted Nextcloud platform. All analytical reports are delivered as fully responsive, multi-tab HTML files.

Complete the form below to receive an example of our ITS2 fungal analysis report and see how we visualize mycobiome profiles for your research